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A2095

Monoclonal Anti-HA−Agarose antibody produced in mouse

Name: Monoclonal Anti-HA−Agarose antibody produced in mouse
Brand: SIGMA

clone HA-7, purified immunoglobulin, PBS suspension

Synonym(s):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD00803873

Conjugate:

agarose conjugate

Clone:

HA-7, monoclonal

Application:

—

Citations:

621

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Properties

biological source

mouse

Quality Level

200

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

PBS suspension

isotype

IgG1

capacity

20-50 nmol/mL elution capacity (HA-tagged fusion protein), 30-50 nmol/mL binding capacity (HA-tagged fusion protein)

shipped in

wet ice

storage temp.

2-8°C

Description

General description

The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.

Biochem/physiol Actions

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Physical form

Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide

Preparation Note

Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品

低风险生物材料

This item has

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Certificates of Analysis (COA)

Lot/Batch Number

0000539178

0000510576