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Merck
CN

A2050

Avidin–FITC from egg white

buffered aqueous solution

Synonym(s):

Avidin–Fluorescein isothiocyanate

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130585
Form:
buffered aqueous solution

Key Documents

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conjugate

FITC conjugate

form

buffered aqueous solution

extent of labeling

3-5.5 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

UniProt accession no.

P02701

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

chicken ... AVD(396260)

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General description

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites.

Application

Avidin-FITC from egg white has been used in:
  • flow cytometry
  • double immunostaining
  • double immunofluorescence
  • immunnohistochemistry

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Physical form

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Preparation Note

Affinity purified protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

动植物来源生物产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000388868
0000312829
0000299666
0000312829
0000299666

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Substance P as a putative efferent transmitter mediates GABAergic inhibition in mouse taste buds
Huang AY and Wu SY
British Journal of Pharmacology, 175(7), 1039-1053 (2018)
Mohamed A Abouelkhair et al.
PloS one, 13(9), e0204450-e0204450 (2018-09-28)
Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair
L Struthers et al.
Analytical biochemistry, 255(1), 20-31 (1998-02-04)
8-Oxodeoxyguanosine is present in DNA from many tissues. The direct demonstration of 8-oxodeoxyguanosine as a potential biomarker of oxidative DNA damage has implications for the study of mutagenesis, carcinogenesis, and free radical toxicity. Avidin is shown here to bind with
The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis
Jain A and Cheng K
Journal of Controlled Release : Official Journal of the Controlled Release Society, 245, 27-40 (2017)
Neuronal uptake of serum albumin is associated with neuron damage during the development of epilepsy
Liu Z, et al.
Experimental and Therapeutic Medicine, 12(2), 695-701 (2016)
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Related Content

Data Sheet - A2050

Instructions

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