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A1888

Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC)

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Synonym(s):
AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
Empirical Formula (Hill Notation):
C18H24N6O6S4
CAS Number:
30931-67-0
Molecular Weight:
548.68
Beilstein:
7329461
EC Number:
250-396-6
MDL number:
MFCD00010404
UNSPSC Code:
12352204
PubChem Substance ID:
24890599
NACRES:
NA.83

Quality Level

200

Assay

≥98% (HPLC)

form

powder

solubility

water: 10 mg/mL, clear to slightly hazy

storage temp.

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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General description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:
  • ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
  • ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

Application

  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
  • ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods.
  • ABTS is also used to monitor glucose concentrations in different solutions including blood serum.
  • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis.
Matsuda H et al.
The Japanese Journal of Experimental Medicine, 54, 131-131 (1984)
Crowther JR
ELISA: Theory and Practice (1995)
Structure-based mutagenesis of herpes simplex virus glycoprotein D defines three critical regions at the gD-HveA/HVEM binding interface.
Connolly SA et al.
Journal of Virology, 77, 8127-8127 (2003)
Expression of glucocorticoid resistance following social stress requires a second signal.
Avitsur R et al.
Journal of Leukocyte Biology, 74, 507-507 (2003)
A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1.
Kramps JA et al.
Journal of Clinical Microbiology, 32, 2175-2175 (1994)
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