A1420
Ames′ Medium
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
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UNSPSC Code:
41161501
NACRES:
NA.75
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L
shipped in
ambient
storage temp.
2-8°C
Related Categories
General description
Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.
Application
Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.
Quantity
Formulated to contain 8.8 grams of powder per liter of medium.
Reconstitution
Supplement with 1.9 g/L sodium bicarbonate.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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B Ameline et al.
Gene therapy, 24(11), 735-741 (2017-09-08)
Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
Michael B Manookin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(16), 4136-4150 (2008-04-18)
Cone signals divide into parallel ON and OFF bipolar cell pathways, which respond to objects brighter or darker than the background and release glutamate onto the corresponding type of ganglion cell. It is assumed that ganglion cell excitatory responses are
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
Frans Vinberg et al.
Vision research, 101, 108-117 (2014-06-25)
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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