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A1355

Sigma-Aldrich

Anti-Archvillin antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, PBS solution

Synonym(s):

Anti-SVIL, Anti-Supervillin (p205/p250), Anti-Supervillin isoform 2, SmAV (Smooth muscle Archvillin)

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About This Item

MDL number:
MFCD07784599
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

PBS solution

mol wt

antigen ~250 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using heat-retrieved tissue sections from human heart by biotin/ExtrAvidin-Peroxidase
indirect immunofluorescence: 20-40 μg/mL using differentiated rat L8 cells
western blot: 2.5-5 μg/mL using whole extract of differentiated mouse C2 cells

UniProt accession no.

O95425

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SVIL(6840)
mouse ... Svil(225115)
rat ... Svil(361256)

General description

Archvillin is a muscle-specific isoform of Supervillin. Archvillin binds F-actin and is believed to be involved in myogenesis and muscle cell migration. Archvillin binds ERK and modulates ERK signaling in smooth cells of the aortas of ferrets.
Rabbit anti-archvillin antibody binds to human, mouse, and rat archvillin.

Immunogen

synthetic peptide corresponding to amino acid residues 650-666 of human archvillin. The corresponding sequence is identical in mouse.

Application

Rabbit anti-archvillin antibody can be used for immunohistochemistry at 20-40 μg/mL using heat-retrieved tissue sections from human heart by biotin/ExtrAvidin-Peroxidase. The antibody can also be used for indirect immunofluorescence at 20-40 μg/mL using differentiated rat L8 cells. Furthermore, the antibody is suitable for use in western blot (2.5-5 μg/mL using whole extract of differentiated mouse C2 cells) assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Samudra S Gangopadhyay et al.
The Journal of biological chemistry, 284(26), 17607-17615 (2009-05-02)
ERK influences a number of pathways in all cells, but how ERK activities are segregated between different pathways has not been entirely clear. Using immunoprecipitation and pulldown experiments with domain-specific recombinant fragments, we show that smooth muscle archvillin (SmAV) binds
Zhiyou Fang et al.
The Journal of biological chemistry, 288(11), 7918-7929 (2013-02-06)
Integrin-based adhesions promote cell survival as well as cell motility and invasion. We show here that the adhesion regulatory protein supervillin increases cell survival by decreasing levels of the tumor suppressor protein p53 and downstream target genes. RNAi-mediated knockdown of
Janelle M Spinazzola et al.
Human molecular genetics, 24(9), 2470-2481 (2015-01-22)
Loss of gamma-sarcoglycan (γ-SG) induces muscle degeneration and signaling defects in response to mechanical load, and its absence is common to both Duchenne and limb girdle muscular dystrophies. Growing evidence suggests that aberrant signaling contributes to the disease pathology; however

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