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Safety Information

A0924

Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 49:1 (ratio)

Synonym(s):

Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution

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About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770639
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

feed ratio

49:1 (ratio)

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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Application

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.
Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PCR Mutation Detection Protocols.
Guilliatt AM
Methods in Molecular Biology (2002)
Bhowmik G
Ana Techniqs in Biotechnology (2011)
Preparation and properties of acrylamide gel for use in electrophoresis.
S RAYMOND et al.
Analytical biochemistry, 1, 391-396 (1960-12-10)

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