A0856
Anti-ATG5 (N-terminal) antibody produced in rabbit
affinity isolated antibody, PBS solution
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Synonym(s):
ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Atg5 Antibody, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
PBS solution
mol wt
antigen ~56 kDa (Atg5-Atg12 complex)
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μL
technique(s)
immunofluorescence: suitable
western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
Related Categories
Specificity
Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.
Immunogen
synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
Application
Anti-ATG5 (N-terminal) antibody has been used:
- in immunoblotting
- in immunofluorescence
- in western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.
Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.
Biochem/physiol Actions
Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis
Grootaert M O A J, et al.
Autophagy, 11(11), 2014-2032 (2015)
Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells
Seto S, et al.
PLoS ONE, 8(12), e86017-e86017 (2013)
Denitsa S Petkova et al.
Viruses, 9(5) (2017-05-23)
Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes
Shintaro Seto et al.
PloS one, 8(12), e86017-e86017 (2014-01-01)
Mycobacterium tuberculosis is an intracellular pathogen that can survive within phagocytic cells by inhibiting phagolysosome biogenesis. However, host cells can control the intracellular M. tuberculosis burden by the induction of autophagy. The mechanism of autophagosome formation to M. tuberculosis has
Kallikrein-8 inhibition attenuates Alzheimer's disease pathology in mice
Herring A, et al.
Alzheimers Dement., 12(12), 1273-1287 (2016)
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