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A0414

Sigma-Aldrich

p-Aminobenzyl 1-thio-β-D-galactopyranoside–Agarose

saline suspension

Synonym(s):

β-Galactopyranoside Agarose, Agarose for Galactopyranoside, Aminobenzyl Agarose

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About This Item

MDL number:
UNSPSC Code:
12161501
NACRES:
NA.32

form

saline suspension

Quality Level

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

12 atoms

capacity

≥10 mg/mL, gel binding capacity (β-galactosidase)

storage temp.

2-8°C

Application

p-Aminobenzyl 1-thio-β-D-galactopyranoside−Agarose has been used in the column for the purification of Bacillus circulans sp. alkalophilus b-galactosidase (Bca-b-gal). It has also been used in the derivatization of affinity matrix of agarose to perform affinity chromatography for the purification of P. haloplanktis β-galactosidase.

Physical form

Suspension in 0.5 M NaCl containing preservative

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Cold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis
Hoyoux A, et al.
Applied and Environmental Microbiology, 67(4), 1529-1535 (2001)
Structural analysis, enzymatic characterization, and catalytic mechanisms of beta-galactosidase from Bacillus circulans sp. alkalophilus
Maksimainen M, et al.
The FEBS journal, 279(10), 1788-1798 (2012)

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