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Sigma-Aldrich

Sephadex® G-25

BioReagent, for molecular biology, DNA grade, Superfine

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About This Item

CAS Number:
9041-35-4
MDL number:
MFCD00132770
UNSPSC Code:
23151817
NACRES:
NA.52

grade

DNA grade
for molecular biology

Quality Level

200

product line

BioReagent

form

beads

technique(s)

buffer exchange: suitable

matrix active group

phase

bead diameter

20-50 μm

pore size

exclusion limit (10 bp dsDNA; (rA)10)

pH range

2-13

application(s)

life science and biopharma

compatibility

Cytiva

foreign activity

DNase, RNase, none detected

storage temp.

room temp

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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General description

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Application

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S K Kim et al.
Journal of agricultural and food chemistry, 49(4), 1984-1989 (2001-04-20)
Gelatin extracted from Alaska pollack skin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The fraction from the second step, which was hydrolyzed with Pronase E, was composed
Veronica Murta et al.
Journal of neurochemistry, 144(6), 748-760 (2017-12-28)
Secondary neuronal death is a serious stroke complication. This process is facilitated by the conversion of glial cells to the reactive pro-inflammatory phenotype that induces neurodegeneration. Therefore, regulation of glial activation is a compelling strategy to reduce brain damage after
Kelsey E Knewtson et al.
ACS omega, 4(7), 12955-12968 (2019-08-29)
Antibody-drug conjugates are an important class of cancer therapeutics. These agents generally bind a specific cell surface receptor, undergo receptor-mediated endocytosis, and enter the endosomal-lysosomal system, where the environment in these organelles facilitates the release of a membrane-permeable cytotoxin. By
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data
Nerine T Joseph et al.
The Journal of endocrinology, 202(1), 179-190 (2009-04-22)
Two GnRH isoforms (cGnRH-I and GnRH-II) and two GnRH receptor subtypes (cGnRH-R-I and cGnRH-R-III) occur in chickens. Differential roles for these molecules in regulating gonadotrophin secretion or other functions are unclear. To investigate this we cloned cGnRH-R-III from a broiler
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