S5772
Sephadex® G-25
BioReagent, DNA grade, Molecular Biology, Superfine
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About This Item
grade
Molecular Biology
Quality Level
product line
BioReagent
form
beads
technique(s)
buffer exchange: suitable
matrix active group
phase
bead diameter
20-50 μm
pore size
exclusion limit (10 bp dsDNA; (rA)10)
pH range
2-13
application(s)
life science and biopharma
compatibility
Cytiva
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI key
BCPVZFFJGCURNR-OCOFDJSDSA-N
General description
Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.
Application
Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Legal Information
Sephadex is a registered trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
S K Kim et al.
Journal of agricultural and food chemistry, 49(4), 1984-1989 (2001-04-20)
Gelatin extracted from Alaska pollack skin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The fraction from the second step, which was hydrolyzed with Pronase E, was composed
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| S5772-100G | 04061833750018 |
| S5772-10G | 04061833750025 |
| S5772-25G | 04061833750032 |