P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Synonym(s):
Proteinase K-Agarose
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About This Item
biological source
microbial (T.album
T. ALBUM)
Quality Level
form
lyophilized powder
extent of labeling
30-140 units per g solid
technique(s)
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
swelling
1 g swells to ~5 mL
storage temp.
−20°C
Application
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Biochem/physiol Actions
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Features and Benefits
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physical form
Lyophilized powder stabilized with lactose
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Shai Eyal et al.
Development (Cambridge, England), 146(14) (2019-06-22)
Bone protrusions provide stable anchoring sites for ligaments and tendons and define the unique morphology of each long bone. Despite their importance, the mechanism by which superstructures are patterned is unknown. Here, we identify components of the genetic program that
Guillaume Saubeau et al.
Molecules (Basel, Switzerland), 19(10), 15374-15390 (2014-09-30)
The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known
Yuan Gao et al.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 31(7), 786-790 (2010-12-18)
To type Klebsiella pneumonia through methods including pulse-field gel electrophoresis (PFGE) in combination with multilocus sequence typing. Four selected different EPs, referring to the Standard Operating Procedure of PulseNet China, were used. The single colony of Klebsiella pneumonia was quantified
M J Pearse et al.
Immunology letters, 18(3), 219-223 (1988-07-01)
This report describes a method for preparing both DNA and RNA simultaneously from as few as 5 X 10(5) lymphoid cells. The method is suitable for cultured cells or any tissue from which a cell suspension can be prepared and
Zohar Erez et al.
Nature, 541(7638), 488-493 (2017-01-19)
Temperate viruses can become dormant in their host cells, a process called lysogeny. In every infection, such viruses decide between the lytic and the lysogenic cycles, that is, whether to replicate and lyse their host or to lysogenize and keep
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