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Key Documents

Safety Information

L0543

Sigma-Aldrich

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with 50 ug/ml kanamycin

Synonym(s):

kanamycin agar plates

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5 G
CN¥387.59
25 G
CN¥1,337.22

CN¥387.59

Available to ship onApril 11, 2025Details

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5 G
CN¥387.59
25 G
CN¥1,337.22

About This Item

UNSPSC Code:
41121800
sterility:
sterile; autoclaved
suitability:
selective for Escherichia coli
selective for coliforms

CN¥387.59

Available to ship onApril 11, 2025Details

grade

Molecular Biology
for molecular biology

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

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This Item
810800P800811C800811O
18:1 DGPP dioleoylglycerol pyrophosphate (ammonium salt), powder

810811P

18:1 DGPP

08:0 DGPP Avanti Research™ - A Croda Brand 810800P, powder

810800P

08:0 DGPP

18:1 DG 1-2-dioleoyl-sn-glycerol, chloroform

800811C

18:1 DG

18:1 DG Avanti Research™ - A Croda Brand

800811O

18:1 DG

packaging

pkg of 1 × 5 mg (810811P-5mg)

packaging

pkg of 1 × 10 mg (810800P-10mg)

packaging

pkg of 1 × 5 mL (800811C-10mg), pkg of 1 × 5 mL (800811C-25mg), pkg of 1 × 8 mL (800811C-200mg)

packaging

pkg of 1 × 10 mg (with screw cap (800811O-10mg)), pkg of 2 × 100 mg (with screw cap (800811O-200mg)), pkg of 5 × 100 mg (with screw cap 800811O-500mg), pkg of 1 × 25 mg (with screw cap (800811O-25mg))

form

powder

form

powder

form

liquid

form

liquid

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

manufacturer/tradename

Avanti Polar Lipids 810811P

manufacturer/tradename

Avanti Polar Lipids 810800P

manufacturer/tradename

Avanti Polar Lipids 800811C

manufacturer/tradename

Avanti Polar Lipids

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

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Description
Pricing

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Kanamycin sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture

L3147

LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

L5667

LB Agar Ampicillin-100, Plates, pre-poured LB agar plates with 100 μg/ml ampicillin

L0418

LB Agar Carbenicillin - 100, Plates, pre-poured agar plates with 100 μg/ml carbenicillin

L0168

LB Agar Ampicillin - 50, Plates, pre-poured LB agar plates with 50 μg/ml ampicillin

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

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OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    MKCW8753
    MKCW8752
    MKCV7424
    MKCV7423
    MKCV7422

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. Why do the LB-agar plates with antibiotics have such a short expiration date?

      The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

    6. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
    Talhinhas P, et al.
    Molecular Biotechnology, 39(1), 57-67 (2008)
    Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
    Deich RA, et al.
    Journal of Bacteriology, 170(2), 489-498 (1988)

    Articles

    Protein Expression Systems

    Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service