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K1377

Kanamycin sulfate from Streptomyces kanamyceticus

powder, BioReagent, suitable for cell culture, suitable for plant cell culture

Synonym(s):

Kanamycin A, Kanamycin sulfate salt

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About This Item

Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
25389-94-0
Molecular Weight:
582.58
NACRES:
NA.76
PubChem Substance ID:
24896204
UNSPSC Code:
51281654
EC Number:
246-933-9
MDL number:
MFCD00070253

Key Documents

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Product Name

Kanamycin sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture

antibiotic activity spectrum

mycobacteria
Gram-negative bacteria
Gram-positive bacteria
mycoplasma

InChI key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

biological source

Streptomyces kanamyceticus

product line

BioReagent

form

powder

potency

≥750 μg per mg (dry basis)

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to off-white

solubility

H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)

application(s)

agriculture

mode of action

protein synthesis | interferes

Quality Level

200

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Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Disclaimer

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

General description

Chemical structure: aminoglycoside
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360D

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000488865
0000488865
0000494666
0000494666
0000462292

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Moxalactam as a counter-selection antibiotic for Agrobacteriummediated
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Plant Science, 164, 607-615 (2003)
Riccardo Di Mambro et al.
Current biology : CB, 29(7), 1199-1205 (2019-03-19)
Plant developmental plasticity relies on the activities of meristems, regions where stem cells continuously produce new cells [1]. The lateral root cap (LRC) is the outermost tissue of the root meristem [1], and it is known to play an important
Samaneh Mosallatpour et al.
Scientific reports, 9(1), 19062-19062 (2019-12-15)
L-glutaminase importance to use in the food industry and medicine has attracted much attention. Enzymes stability has always been a challenge while working with them. We heterologously expressed and characterized a novel stable L-glutaminase from an extremophile bacterium (Cohnella sp.
Atef Allam et al.
Journal of immunology (Baltimore, Md. : 1950), 193(2), 871-878 (2014-06-11)
The role of the TNF family member CD70 in adaptive T cell responses has been intensively studied, but its function in innate responses is still under investigation. In this study, we show that CD70 inhibits the early innate response to
Mitsutaka Kitano et al.
Antimicrobial agents and chemotherapy, 58(8), 4795-4803 (2014-06-11)
Highly pathogenic avian influenza A (H5N1) viruses cause severe and often fatal disease in humans. We evaluated the efficacy of repeated intravenous dosing of the neuraminidase inhibitor peramivir against highly pathogenic avian influenza virus A/Vietnam/UT3040/2004 (H5N1) infection in cynomolgus macaques.
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Product Information Sheet - K1377

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