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I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352205
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sterility

sterile-filtered

Quality Level

500

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000425206
0000425206
0000416500
0000416500
0000366647

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Articles

Selenium in Cell Culture

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

细胞培养中的转铁蛋白

转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。本页介绍转铁蛋白的体外化学和生物化学。

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Global Trade Item Number

SKUGTIN
I3146-5ML04061838108593