H7140
Hybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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UNSPSC Code:
12161700
NACRES:
NA.25
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Quality Level
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
Related Categories
General description
Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.
Application
Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.
Components
Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
related product
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
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Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Jürgen R Sindermann et al.
American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
Joel W Slaton et al.
Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
Protein serine/threonine kinase CK2 (formerly casein kinase 2) is a ubiquitous protein kinase that plays key roles in cell growth, proliferation, and survival. We have shown previously that its molecular down-regulation induces apoptosis in cancer cells in culture. Here, we
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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