Skip to Content

For important updates on recent policy changes, please click here for more details.

Merck
CN
All Photos(1)

Key Documents

Safety Information

H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

Sign Into View Organizational & Contract Pricing

Select a Size

10 G
¥383.71
25 G
¥509.14
100 G
¥1,768.94

¥383.71

Available to ship on2025年4月10日Details

All Photos(1)

Select a Size

Change View
10 G
¥383.71
25 G
¥509.14
100 G
¥1,768.94

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

¥383.71

Available to ship on2025年4月10日Details

Quality Level

100

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
TZHALA210TZHVMD210TZHVAB205
Steritest® vent needles suitable for sterility testing, for glass vials with rubber septa and rigid plastic vials., PTFE (hydrophobic membrane filter)

TEFG02525

Steritest® vent needles

Steritest® NEO Device For liquids in ampoules or collapsible bags., Blue base canister with a single-needle adapter., pkg of 10 blisters per box, Single packed

TZHALA210

Steritest® NEO Device

Steritest® NEO Device For medical devices and collapsible bags., Red base canister provided with 3 needle adapters to connect to a variety of test devices., pkg of 10 blisters per box, Single packed

TZHVMD210

Steritest® NEO Device

Steritest® NEO Device For liquids in ampoules and collapsible bags., Red base canister with single needle for easy access., pkg of 10 blisters in 2 bags of 5 blisters per box, Double packed

TZHVAB205

Steritest® NEO Device

application(s)

pharmaceutical, sterility testing

application(s)

pharmaceutical
sterility testing

application(s)

pharmaceutical
sterility testing

application(s)

pharmaceutical
sterility testing

pore size

0.2 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

packaging

pkg of 25 units

packaging

pkg of 10 blisters per box, Single packed

packaging

pkg of 10 blisters per box, Single packed

packaging

pkg of 10 blisters in 2 bags of 5 blisters per box, Double packed

material

PTFE (hydrophobic membrane filter), stainless steel (for needle), PVC device

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), mixed cellulose esters (MCE) membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), PVDF membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), PVDF membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Application

Hybridization Solution has been used to hybridise the nitrocellulose membrane.[1] It has also been used in DNA hybridization.[2]
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Components

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCM9514
SLCC2282
SLBR7356V
SLBP5108V
SLBH6970

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Silicon nanonets for biological sensing applications with enhanced optical detection ability
Serre P, et al.
Biosensors And Bioelectronics, 68, 336-342 (2015)
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
Jürgen R Sindermann et al.
American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
Joel W Slaton et al.
Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
Protein serine/threonine kinase CK2 (formerly casein kinase 2) is a ubiquitous protein kinase that plays key roles in cell growth, proliferation, and survival. We have shown previously that its molecular down-regulation induces apoptosis in cancer cells in culture. Here, we
View All Related Papers

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service