D1556
OptiPrep™ Density Gradient Medium
used for cell and subcellular organelle isolation
Synonym(s):
Iodixanol solution
About This Item
Quality Level
sterility
sterile
form
liquid
density
1.319-1.321 g/mL
application(s)
hematology
histology
SMILES string
CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I
InChI
1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)
InChI key
NBQNWMBBSKPBAY-UHFFFAOYSA-N
General description
Application
- to investigate the efficiency of single-cell encapsulation.
- the gradient-based isolation of senescent cancer cells.
- isolation of non-enveloped virion isolation from bronchoalveolar lavage.
- isolation of lipoproteins and macromolecules
- purification of virions
- purification of neuron cells
Features and Benefits
- It is biologically inert and does not interfere with assays for marker enzymes.
- It is non-ionic, non-toxic, and has low osmotic pressure.
- It forms spontaneous gradients that are stabler and easier to prepare than sucrose.
Other Notes
Legal Information
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?
Percoll and Histopaque must be removed prior to use through washing. If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells. In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.
What is the appearance of Product D1556, OptiPrep?
The solution may be clear to slightly yellow.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?
After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.
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