94498
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride
≥85% (HPLC)
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Synonym(s):
5-Cyano-2,3-bis(4-methylphenyl)-2H-tetrazolium chloride, CTC
Empirical Formula (Hill Notation):
C16H14ClN5
CAS Number:
Molecular Weight:
311.77
Beilstein:
8954451
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Recommended Products
Quality Level
Assay
≥85% (HPLC)
form
powder
storage temp.
2-8°C
SMILES string
[Cl-].Cc1ccc(cc1)-n2nc(n[n+]2-c3ccc(C)cc3)C#N
InChI
1S/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15;/h3-10H,1-2H3;1H/q+1;/p-1
InChI key
SSJZAXOTLCJNLF-UHFFFAOYSA-M
Related Categories
General description
5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride is a Redox Sensitive tetrazolium salt producing a fluorescent formazan (only in solid state) upon reduction; It indicates the respiratory activity in cells.
Application
5-cyano-2,3-di(p-tolyl) tetrazolium chloride (CTC)-reduction test is used for live/dead analysis. 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride has been employed in histochemistry and for determining cell viability, for eukaryotic cell viability assays and bacterial viability experiments, 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) is the most often employed compound.
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Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Juan C Stockert et al.
Acta histochemica, 120(3), 159-167 (2018-03-03)
For many years various tetrazolium salts and their formazan products have been employed in histochemistry and for assessing cell viability. For the latter application, the most widely used are 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) for viability assays of
Severin, E., et al.
Analytica Chimica Acta, 170, 341-341 (1985)
Rodriguez, G.G., et al.
Applied and Environmental Microbiology, 58, 180-180 (1992)
Yuta Morishige et al.
Biological & pharmaceutical bulletin, 37(10), 1617-1625 (2014-08-12)
An environmental isolate of Salmonella enterica serovar Enteritidis (SE) clone, SE Cl#15-1, loses its culturability during 72-h culture in M9 minimal medium containing 0.8% glucose, a concentration twice higher than that in normal M9 medium, whereas the bacterium retains its
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