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93759

Sigma-Aldrich

Pyridoxal hydrochloride

≥99.5% (T)

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Synonym(s):
3-Hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridinecarboxaldehyde hydrochloride, PL HCl
Empirical Formula (Hill Notation):
C8H9NO3 · HCl
CAS Number:
65-22-5
Molecular Weight:
203.62
Beilstein:
3656994
EC Number:
200-602-5
MDL number:
MFCD00012809
UNSPSC Code:
12352200
PubChem Substance ID:
329770265
NACRES:
NA.79

biological source

synthetic

Quality Level

300

Assay

≥99.5% (T)

form

powder

loss

≤0.6% loss on drying

color

white to faint beige

mp

173 °C (dec.) (lit.)

anion traces

sulfate (SO42-): ≤1000 mg/kg

cation traces

Ca: ≤250 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤15 mg/kg
Cu: ≤5 mg/kg
Fe: ≤15 mg/L
K: ≤50 mg/kg
Mg: ≤10 mg/kg
Mn: ≤100 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

cell analysis

SMILES string

Cl[H].[H]C(=O)c1c(CO)cnc(C)c1O

InChI

1S/C8H9NO3.ClH/c1-5-8(12)7(4-11)6(3-10)2-9-5;/h2,4,10,12H,3H2,1H3;1H

InChI key

FCHXJFJNDJXENQ-UHFFFAOYSA-N

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General description

Pyridoxal is a derivative of vitamin B6. Vitamin B6 derivatives contain a core pyridine ring. Pyridoxal consists of an aldehyde group at pyridine′s 4′ position. Pyridoxal is a water-soluble vitamin.

Application

For the labeling of amino acids and their detection in picomolar amounts; Coenzymes and Cofactors, vol. 1: Vitamin B6, Pyridoxal Phosphate

Biochem/physiol Actions

Vitamin B6 derivatives are initially oxidized to pyridoxal. It is then phosphorylated to pyridoxal 5′- phosphate (P5P). Pyridoxal 5′- phosphate is the circulatory form of vitamin B6.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of water-soluble vitamins in infant milk by high-performance liquid chromatography
Albala S, et al.
Journal of Chromatography A, 778(1-2), 247-253 (1997)
Thomas Bachmann et al.
Analytical and bioanalytical chemistry, 412(26), 7237-7252 (2020-08-17)
Vitamin B6 comprises an important set of molecules tightly interwoven with the human amino acid, fatty acid, and carbohydrate metabolism. Analytical methods striving for the quantification of individual B6 vitamers so far mostly rely on methods based on HPLC in
Vitamin B6 (Pyridoxine; Pyridoxal 5'-Phosphate)
CH, C
Alternative Medicine Review, 6(1), 87-87 (2001)
Vitamin B6 and its role in cell metabolism and physiology
Parra M, et al.
Cells, 7(7), 84-84 (2018)
Toshio Iwasaki et al.
Journal of the American Chemical Society, 131(38), 13659-13667 (2009-09-10)
CW EPR spectra of reduced [2Fe-2S](Cys)(3)(His)(1) clusters of mammalian mitoNEET soluble domain appear to produce features resulting from the interaction of the electron spins of the two adjacent clusters, which can be explained by employing the local spin model. This
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