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Merck
CN

93711

Atto 655

BioReagent, suitable for fluorescence, ≥85% (HPLC)

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About This Item

UNSPSC Code:
12352116
PubChem Substance ID:
57653573
NACRES:
NA.32
MDL number:
MFCD03456136

Key Documents

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Product Name

Atto 655, BioReagent, suitable for fluorescence, ≥85% (HPLC)

SMILES string

[S](=O)(=O)([O-])CC1CC(N(c2c1cc3c(c2)OC4=CC5=[N+](CCCC5=CC4=N3)CC)CCCC(=O)O)(C)C

InChI

1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

FOYVTVSSAMSORJ-UHFFFAOYSA-N

product line

BioReagent

assay

≥85% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
655 nm

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 652-658 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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Application

Atto labels are designed for highest sensitivity applications. A unique combination of advantages makes them highly favorable tools for all kinds of labeling applications. Some of their properties make them specifically interesting for single molecule detection. Atto labels are based on rigid structures and do not show any cis-trans-isomerization, which lowers the brightness of signals and leads to environment dependency, e.g., spectral shifts by conjugation.
Atto 655 shows a molar extinction of 110,000 and QY of 30% in water (50% in ethanol). Decay time is 1.9 ns.

Other Notes

New red absorbing fluorescent dye with best signal-to-noise ratio and long fluorescence life-time. Useful as a biophysical probe for binding interactions.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0768
BCCN8603
BCCN5830
BCCN4662
BCCN3519

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Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Bengang Xing et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(50), 14170-14177 (2011-11-16)
The molecular interactions of the glycopeptide antibiotic vancomycin (Van) with bacterial cell wall analogues N,N'-diacetyl-L-Lys-D-Ala-D-Ala (Ac(2) KdAdA) and N,N'-diacetyl-L-Lys-D-Ala-D-Lac (Ac(2) KdAdL) were investigated in neat water, phosphate buffer and HEPES buffer by using fluorescence correlation spectroscopy (FCS) and molecular dynamics
Fabian Heinemann et al.
Langmuir : the ACS journal of surfaces and colloids, 28(37), 13395-13404 (2012-08-16)
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients of lipids and proteins in biological membranes. In recent years, several variants of FCS have been introduced. However, a comprehensive comparison of these methods on identical systems
Katharina Stöhr et al.
Analytical chemistry, 77(22), 7195-7203 (2005-11-16)
Due to growing problems with new emerging pathogens, cost-effective and manageable methods for their accurate identification in routine diagnostics are urgently required. Of particular importance is the genus Mycobacterium with its more than 100 species. Identification of these species is
Zhixing Chen et al.
Journal of the American Chemical Society, 134(33), 13692-13699 (2012-08-10)
Chemical tags are now viable alternatives to fluorescent proteins for labeling proteins in living cells with organic fluorophores that have improved brightness and other specialized properties. Recently, we successfully rendered our TMP-tag covalent with a proximity-induced reaction between the protein
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