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93363

Sigma-Aldrich

Trizma® hydrochloride

BioUltra, ≥99.0% (AT)

Synonym(s):

TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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100 G
CN¥390.72
500 G
CN¥1,527.06

CN¥390.72

Available to ship onApril 14, 2025Details

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100 G
CN¥390.72
500 G
CN¥1,527.06

About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
UNSPSC Code:
12161700
PubChem Substance ID:
57653541
NACRES:
NA.25

CN¥390.72

Available to ship onApril 14, 2025Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

product line

BioUltra

Assay

≥99.0% (AT)

form

powder or crystals

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.2% (as SO4)

loss

≤0.2% loss on drying, 110 °C

pH

2.5-4.0 (25 °C, 4 M in H2O)

useful pH range

7.0-9.0

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Show Differences

1 of 4

This Item
481907N3376BP460
烟酰胺 BioReagent, suitable for cell culture, suitable for insect cell culture

N0636

烟酰胺

Nicotinamide Precursor of the coenzymes NAD+ and NADP+.

481907

Nicotinamide

烟酰胺 ≥98% (HPLC), powder

N3376

烟酰胺

烟酰胺 British Pharmacopoeia (BP) Reference Standard

BP460

烟酰胺

assay

≥98% (HPLC)

assay

≥99% (HPLC)

assay

≥98% (HPLC)

assay

-

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

form

powder

form

solid

form

powder

form

solid

solubility

water: soluble 50 mg/mL, clear, colorless

solubility

water: soluble

solubility

-

solubility

-

mp

128-131 °C (lit.)

mp

-

mp

128-131 °C (lit.)

mp

128-131 °C (lit.)

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

Component of extraction buffer e.g. phenol extraction of DNA or RNAcitation; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGEcitation.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Component of extraction buffer e.g. phenol extraction of DNA or RNA[1]; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE[2].

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCN0127
    BCCM7499
    BCCM1820
    BCCM5867
    BCCK3734

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    Santiago Vilanova et al.
    Plant methods, 16, 110-110 (2020-08-15)
    The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic DNA (gDNA) in sufficient concentrations is often a main limitation, especially for
    D Sirieix et al.
    American journal of respiratory and critical care medicine, 155(3), 957-963 (1997-03-01)
    Metabolic acidosis induces a decrease in the developed force of cardiac muscle by affecting every step of the excitation--contraction coupling pathway. Due to transient worsening in intracellular acidosis, the value of administering sodium bicarbonate therapeutically during acute acidosis has been
    G G Nahas et al.
    Drugs, 55(2), 191-224 (1998-03-20)
    THAM (trometamol; tris-hydroxymethyl aminomethane) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. At 37 degrees C, the pK (the pH at which the weak conjugate acid or base
    Characterization of in vitro translation products.
    R Jagus
    Methods in enzymology, 152, 296-304 (1987-01-01)
    Large- and small-scale phenol extractions.
    D M Wallace
    Methods in enzymology, 152, 33-41 (1987-01-01)
    View All Related Papers

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