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Trizma^® base

Name: Trizma<SUP>®</SUP> base
Brand: SIGMA

≥99.7% (T), crystalline, puriss p.a., aminopeptidase substrate

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

Beilstein:

741883

EC Number:

201-064-4

MDL number:

MFCD00004679

UNSPSC Code:

12352104

PubChem Substance ID:

57653537

NACRES:

NA.25

product name

Trizma^® base, puriss. p.a., ≥99.7% (T)

description

aminopeptidase substrate

Quality Level

200

grade

puriss. p.a.

Assay

≥99.7% (T)

form

crystalline

ign. residue

≤0.01% (as SO₄)

pH

10.5-11.5 (25 °C, 10% in H₂O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

water: soluble (678 g/l at 20 °C)

anion traces

chloride (Cl^-): ≤5 mg/kg
sulfate (SO₄^2-): ≤5 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

10 % in H₂O

UV absorption

λ: 230 nm A_max: ≤0.6
λ: 260 nm A_max: ≤0.05
λ: 280 nm A_max: ≤0.04
λ: 430 nm A_max: ≤0.01

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Related Categories

Biological Buffers

Tris

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
As a component of sample buffer for SDS-PAGE
In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.

Description

Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay.

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Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.

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Preparation of Buffers for Use in Enzyme Studies.

Gomori G

Methods in Enzymology, 1, 138-146 (1955)

Tri (hydroxymethyl) aminomethane as Acidimetric Standard.

Fossum J, et al.

Analytical Chemistry, 23(3), 491-493 (1951)

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