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Merck
CN

93350

Trizma® base

99.7% (T), puriss. p.a.

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
UNSPSC Code:
12352104
NACRES:
NA.25
PubChem Substance ID:
57653537
EC Number:
201-064-4
Beilstein/REAXYS Number:
741883
MDL number:
MFCD00004679

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Product Name

Trizma® base, 99.7% (T), puriss. p.a.

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO

description

aminopeptidase substrate

grade

puriss. p.a.

form

crystalline

ign. residue

≤0.01% (as SO4)

pH

10.5-11.5 (25 °C, 10% in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

water: soluble (678 g/l at 20 °C)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

10 % in H2O

UV absorption

λ: 230 nm Amax: ≤0.6
λ: 260 nm Amax: ≤0.05
λ: 280 nm Amax: ≤0.04
λ: 430 nm Amax: ≤0.01

Quality Level

200

assay

99.7% (T)

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Application

Tris base has been used:
  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6291
BCCL5306
BCCM3159
BCCK1131
BCCJ4403

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Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62(1), 61-66 (2002)
In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay.
Liu Y, et al.
Journal of Bacteriology (2017)
a-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay.
Wordehoff M and Hoyer W
Bio-protocol, 8(14) (2018)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Sambrook J and Russell DW
Molecular Cloning: A Laboratory Manual (2001)
View All Related Papers

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Trizma Base Specification Comparison

Trizma Base Specification Comparison

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