All Photos(1)

Documents

92846

5-Carboxyfluorescein N-succinimidyl ester

Name: 5-Carboxyfluorescein <I>N</I>-succinimidyl ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sign Into View Organizational & Contract Pricing

All Photos(1)

About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₅NO₉

CAS Number:

92557-80-7

Molecular Weight:

473.39

Beilstein:

7865828

MDL number:

MFCD00077322

UNSPSC Code:

12352108

PubChem Substance ID:

57653496

NACRES:

NA.32

product line

BioReagent

Quality Level

100

Assay

≥90% (HPLC)

form

powder

fluorescence

λ_ex 492 nm; λ_em 520 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides; Reagent for fluorescent derivatization in capillary electrophoresis

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nonradioactive labeling of RNA.

G L Igloi

Analytical biochemistry, 233(1), 124-129 (1996-01-01)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of

Lau, S.K., et al.

Journal of Chromatography A, 809, 203-203 (1998)

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

L G Lee et al.

Nucleic acids research, 20(10), 2471-2483 (1992-05-25)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments

Monocyte adhesion to atherosclerotic matrix proteins is enhanced by Asn-Gly-Arg deamidation.

Bamaprasad Dutta et al.

Scientific reports, 7(1), 5765-5765 (2017-07-20)

Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation

A DNA-based molecular probe for optically reporting cellular traction forces.

Brandon L Blakely et al.

Nature methods, 11(12), 1229-1232 (2014-10-13)

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service