92011
Nitrile hydratase
recombinant, expressed in E. coli, ≥1.5 U/mg
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About This Item
CAS Number:
UNSPSC Code:
12352204
recombinant
expressed in E. coli
form
lyophilized
specific activity
≥1.5 U/mg
storage temp.
−20°C
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Application
Nitrile hydratase is an enzyme that is used to convert nitriles to amides. It is used to study the metabolism of nitriles in bacteria, to produce acrylamide, and to remove nitriles from wastewater . Product 92011 is used to convert acrylonitrile to acrylamide.
Biochem/physiol Actions
Nitrile hydratase catalyses the hydration of nitriles to their corresponding amides. Nitrile hydratase is composed of α- and β-subunits. It has a non-heme iron or a noncorrin cobalt ion in a ligand environment that includes two oxidized cysteine residues .
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
1 U corresponds to the amount of enzyme which converts 1 μmol acrylonitrile to acrylamide per minute at 30°C and pH 7.2
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Zhemin Zhou et al.
The Journal of biological chemistry, 284(22), 14930-14938 (2009-04-07)
The incorporation of cobalt into low molecular mass nitrile hydratase (L-NHase) of Rhodococcus rhodochrous J1 has been found to depend on the alpha-subunit exchange between cobalt-free L-NHase (apo-L-NHase lacking oxidized cysteine residues) and its cobalt-containing mediator (holo-NhlAE containing Cys-SO(2)(-) and
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