91252
2-Deoxyribose 5-phosphate Aldolase, E. coli K12, recombinant K12 from E. coli
≥2 U/mg
Synonym(s):
DERA, Deoxyriboaldolase
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About This Item
CAS Number:
Enzyme Commission number:
4.1.2.4.
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
crystals
powder
specific activity
≥2 U/mg
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Deoxyriboaldolase catalyzes the condensation of acetaldehyde and glyceraldehyde 3-phosphate to 2-deoxyribose 5-phosphate.
Unit Definition
1 U corresponds to the amount of enzyme which converts 1 μmol 2-Deoxy-D-ribose-5-phosphate per minute at pH 7.5 and 25°C
Other Notes
The enzyme catalyzes the reversible cleavage of 2-deoxyribose-5-phosphate to acetaldehyde and D-glyceraldehyde-3-phosphate
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Xiaolin Pei et al.
Applied biochemistry and biotechnology, 162(5), 1423-1434 (2010-03-17)
2-Deoxyribose-5-phosphate aldolase (DERA) catalyzes a sequential aldol reaction useful in synthetic chemistry. In this work, the effect of a feeding strategy on the production of a thermophilic DERA was investigated in fed-batch cultures of recombinant Escherichia coli BL21 (pET303-DERA008). The
F Sgarrella et al.
Biochimica et biophysica acta, 1118(2), 130-133 (1992-01-09)
Deoxyribose 5-phosphate aldolase was purified 41 times from Bacillus cereus induced by growth on deoxyribonucleosides. The purification procedure includes ammonium sulphate fractionation, gel filtration on Sephadex G-100, ion-exchange chromatography on DEAE-Sephacel and preparative electrophoresis on 10% polyacrylamide gel. The enzyme
X Zeng et al.
Journal of bacteriology, 181(6), 1719-1727 (1999-03-12)
The deoR gene located just upstream the dra-nupC-pdp operon of Bacillus subtilis encodes the DeoR repressor protein that negatively regulates the expression of the operon at the level of transcription. The control region upstream of the operon was mapped by
Akio Ueno et al.
Molecular and biochemical parasitology, 173(1), 39-42 (2010-05-04)
The Toxoplasma gondii deoxyribose phosphate aldolase-like (TgDPA) gene is expressed predominantly in bradyzoites. This finding allowed us to infer that TgDPA is important in the tachyzoite-to-bradyzoite development or maintenance of cyst structure although the function of this gene is still
Nobuyuki Horinouchi et al.
Applied and environmental microbiology, 69(7), 3791-3797 (2003-07-04)
The gene encoding a deoxyriboaldolase (DERA) was cloned from the chromosomal DNA of Klebsiella pneumoniae B-4-4. This gene contains an open reading frame consisting of 780 nucleotides encoding 259 amino acid residues. The predicted amino acid sequence exhibited 94.6% homology
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