Skip to Content
Merck
CN
All Photos(1)

Documents

88417

Sigma-Aldrich

MTT Formazan

MTT reduction end product, powder

Synonym(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
57360-69-7
Molecular Weight:
335.43
MDL number:
MFCD00080731
UNSPSC Code:
12352204
PubChem Substance ID:
329769650
NACRES:
NA.83

product name

MTT Formazan, MTT reduction end product

Quality Level

100

form

powder

solubility

chloroform: 10 mg/mL, clear, dark violet

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

Looking for similar products? Visit Product Comparison Guide

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

related product

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Chao Zhang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 80, 253-259 (2016-05-03)
Costunolide, an active sesquiterpene lactone, is derived from many herbal medicines and it exhibits a broad spectrum of bioactivities such as anti-inflammatory, potential anti-tumor activity. Herein we assessed the anti-cancer effects of costunolide on U2OS cells and explored the underlying
Jianing Li et al.
Pathology, research and practice, 215(10), 152591-152591 (2019-08-26)
The role of transforming growth factor beta (TGF-β) in lung cancer is well known. TGF-β-mediated cellular proliferation and angiogenesis through similar to mothers against decapentaplegic homolog 2 (Smad2) protein has also been well elucidated. Smad2 is a predicted target for
Polymeric nanoparticles for the selective therapy of inflammatory bowel disease
Test, 508, 377-397 (2012)
Xi Jiang et al.
Medical science monitor : international medical journal of experimental and clinical research, 26, e924507-e924507 (2020-08-01)
BACKGROUND Curcumin derivative C086 (cur C086) is a potential chemotherapeutic agent for patients with osteosarcoma. In this study, the effects of cur C086 combined with cisplatin on the biological processes of osteosarcoma cells were investigated. MATERIAL AND METHODS In this
Primary cultures of cardiac myocytes as in vitro models for pharmacological and toxicological assessments
In Vitro Methods in Pharmaceutical Research, 209-223 (1997)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service