Skip to Content
Merck
CN

86826

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

5-FAM

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57653195
MDL number:
MFCD00036874
Beilstein/REAXYS Number:
57037

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

5-Carboxyfluorescein, BioReagent, suitable for fluorescence, ≥95% (HPLC)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

product line

BioReagent

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

Quality Level

100

Looking for similar products? Visit Product Comparison Guide

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM9319
BCCJ6763
BCCD5220
BCBZ5682
BCBW2017

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The loss-of-allele assay for ES cell screening and mouse genotyping
Methods in Enzymology, 476, 295-307 (2010)
Intracellular pH and its Measurement (1989)
Detection of Bacteria, Viruses, Parasites and Fungi (2010)
Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes
Hudiyanti D, et al.
Orient. J. Chem., 31, 435-439 (2015)
Peilin Yang et al.
Analytical chemistry, 77(8), 2482-2489 (2005-04-15)
Fluorescence anisotropy capillary electrophoresis (FACE) and affinity probe capillary electrophoresis (APCE) with laser-induced fluorescence detection were evaluated for analysis of peptide-protein interactions with rapid binding kinetics. The Src homology 2 domain of protein SH2-Bbeta (SH2-Bbeta (525-670)) and a tyrosine-phosphorylated peptide
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service