85878
Streptavidin from Streptomyces avidinii
affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein
Product Name
Streptavidin from Streptomyces avidinii, affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein
form
powder
quality
affinity purified
lyophilized from 10 mM potassium phosphate
specific activity
≥13 U/mg protein
mol wt
Mr ~60000
solubility
H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow
storage temp.
−20°C
Quality Level
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Analysis Note
Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.
Application
Streptavidin from Streptomyces avidinii has been used:
- in the pre-functionalization of self-propelled catalytic micromotors
- in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
- as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)
Biochem/physiol Actions
Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.
General description
Streptavidin is a crystalline protein synthesized by Streptomycetes.
Other Notes
1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5
Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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D.A. Fuccillo
Biotechniques, 3, 494-494 (1985)
Scientific and Clinical Applications of Magnetic Carriers (2013)
Soon Gang Choi et al.
Nature communications, 10(1), 3907-3907 (2019-08-31)
Complementary assays are required to comprehensively map complex biological entities such as genomes, proteomes and interactome networks. However, how various assays can be optimally combined to approach completeness while maintaining high precision often remains unclear. Here, we propose a framework
Yuan-Yuan Chen et al.
Journal of Cancer, 7(1), 69-79 (2016-01-02)
Capture and identification of circulating tumor cells (CTCs) in the blood system can help guide therapy and predict the prognosis of cancer patients. However, simultaneous capture and identification of CTCs with both epithelial and mesenchymal phenotypes remains a formidable technical
Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding
Magalhaes M L B, et al.
Protein Science, 20(7), 1145-1154 (2011)
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