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Sigma-Aldrich

Rhodamine B

for fluorescence

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
81-88-9
Molecular Weight:
479.01
Colour Index Number:
45170
Beilstein:
4091619
EC Number:
201-383-9
MDL number:
MFCD00011931
UNSPSC Code:
12171500
PubChem Substance ID:
57652962
NACRES:
NA.32

grade

for fluorescence

Quality Level

200

form

powder or crystals

mp

210-211 (dec.) (lit.)

solubility

H2O: soluble
methanol: soluble

fluorescence

λex 553 nm; λem 627 nm in methanol

suitability

in accordance for fluorescence

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

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General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
Rhodamine B is bright-red in color.

Application

Rhodamine B has been used:
  • as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
  • as a fluorescent dye in confocal microscopy
  • to study its fluorescence in water and ethanol solutions

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)

Analysis Note

λmax. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H318,H412

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rhodamine B as a probe for phase transition in liquid solutions
Shishkin I, et al.
arXiv (2016)
The fluorescence properties of cationic rhodamine B in the gas phase
Sagoo SK and Jockusch RA
Journal of Photochemistry and Photobiology A: Chemistry, 220(2-3), 173-178 (2011)
Simplifying microfluidic separation devices towards field-detection of blood parasites
Holm SH, et al.
Analytical Methods : Advancing Methods and Applications, 8(16), 3291-3300 (2016)
MICAN, a new fluorophore for vital and non-vital staining of human cells
Nagy Z, et al.
Toxicology in vitro, 48, 137-145 (2018)
The
A study of water circulation in Monterey harbor using rhodamine B dye (1965)
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