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83200

Sigma-Aldrich

Pyronin Y

for microscopy (Bot., Fl., Hist.)

Synonym(s):

Pyronin G, Pyronin J

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About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12171500
PubChem Substance ID:
57652949
NACRES:
NA.47

grade

for microscopy (Bot., Fl., Hist.)

Quality Level

100

mp

250-260 °C (lit.)

solubility

water: 0.01 g/10 mL, red

εmax

≥1650 at 543-553 nm in 50% ethanol
≥650 at 249-259 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ8731
BCCC6788
BCBW9213
BCBS0287V
BCBK5183V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?

    No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable.  Product No. P9172 has been used to stain RNA in polyacrylamide gels.  Product No. 213519 was submitted to the Biological Stain Commission for certification.  Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.

  6. Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?

    Yes, product HT70116 is available in pre-made form.  The product is approved for in vitro diagnostic (IVD) use.

  7. What is the emission wavelength of Pyronin Y, Product 83200?

    The emission is at 570 nm when dissolved in water.

  8. What is the solubility of Pyronin Y, Product 83200, in water and ethanol?

    Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Rost FWD
Fluorescence Microscopy (1995)
Karsten Kuritz et al.
Scientific reports, 10(1), 3619-3619 (2020-02-29)
Modern cytometry methods allow collecting complex, multi-dimensional data sets from heterogeneous cell populations at single-cell resolution. While methods exist to describe the progression and order of cellular processes from snapshots of such populations, these descriptions are limited to arbitrary pseudotime
Pedro Montes-Navajas et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(12), 1743-1747 (2009-12-22)
Cucurbiturils (CBs) of the appropriate size (CB[7] and CB[8]) form strong guest-host complexes in phosphate buffer solution (PBS) with acridine orange (AO) and pyronine Y (PYY) with 1 : 1 and 2 : 1 stoichiometries for CB[7] and CB[8] complexes
Yuichiro Koide et al.
ACS chemical biology, 6(6), 600-608 (2011-03-08)
The absorption and emission wavelengths of group 14 pyronines and rhodamines, which contain silicon, germanium, or tin at the 10 position of the xanthene chromophore, showed large bathochromic shifts compared to the original rhodamines, owing to stabilization of the LUMO
Myron Nevins et al.
Journal of periodontology, 83(7), 878-884 (2012-01-10)
This study tests the effectiveness of hydroxyapatite and collagen bone blocks of equine origin (eHAC), infused with recombinant human platelet-derived growth factor-BB (rhPDGF-BB), to augment localized posterior mandibular defects in non-human primates (Papio hamadryas). Bilateral critical-sized defects simulating severe atrophy
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