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79636

Supelco

Atto 590 NHS ester

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

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1 MG
CN¥1,629.02
5 MG
CN¥10,009.94

CN¥1,629.02

Estimated to ship onApril 07, 2025Details

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1 MG
CN¥1,629.02
5 MG
CN¥10,009.94

About This Item

Empirical Formula (Hill Notation):
C41H42ClN3O11
CAS Number:
670269-33-7
Molecular Weight:
788.24
MDL number:
MFCD03456133
UNSPSC Code:
12352108
NACRES:
NA.32

CN¥1,629.02

Estimated to ship onApril 07, 2025Details

Request a Bulk Order

product line

BioReagent

Assay

≥90.0% (HPLC)
≥90.0% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0,1% trifluoroacetic acid)

UV absorption

λ: 597-663 nm Amax

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

SMILES string

[Cl](=O)(=O)(=O)[O-].[N+]1(=C2C(=CC3=C(c6c(cc7c(c6)C(=CC(N7CC)(C)C)C)OC3=C2)c4c(ccc(c4)C(=O)ON5C(=O)CCC5=O)C(=O)O)C(=CC1(C)C)C)CC

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This Item
674559399078362
Atto 590 NHS ester BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

79636

Atto 590 NHS ester

Sigma-Aldrich

67455

Atto 514 NHS ester

Sigma-Aldrich

93990

Atto 590 alkyne

Sigma-Aldrich

78362

Atto 490LS NHS ester

form

powder

form

solid

form

powder

form

solid

assay

≥90.0% (HPLC), ≥90.0% (degree of coupling)

assay

90% (Coupling efficiency with benzylamine, HPLC)

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling)

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

product line

BioReagent

product line

-

product line

BioReagent

product line

BioReagent

General description

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

Application

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.[1]
Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data)

Features and Benefits

  • Absorption maxima cover a range from 590 to 620 nm.
  • Structural properties support extraordinary photostability.
  • High fluorescence quantum yield and high thermal stability.
  • The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
  • Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Other Notes

Study of multistep energy transfer in a single photonic wire by FRET[2]; employed in fluorescence energy transfer, FRET, scanning microscopy[3].

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM6572
    BCCM6582
    BCCM0700
    BCCL7625
    BCCL4403

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    Y. Eberstein et al.
    J. Chem. Phys. , B 108, 93-93 (2004)
    Handbook of fluorescence spectroscopy and imaging: from ensemble to single molecules null
    D Wildanger et al.
    Journal of microscopy, 236(1), 35-43 (2009-09-24)
    The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
    Mike Heilemann et al.
    Journal of the American Chemical Society, 126(21), 6514-6515 (2004-05-27)
    We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various
    Martin Baumdick et al.
    Nature communications, 9(1), 3847-3847 (2018-09-23)
    Epidermal growth factor receptor (EGFR) activation by growth factors (GFs) relies on dimerization and allosteric activation of its intrinsic kinase activity, resulting in trans-phosphorylation of tyrosines on its C-terminal tail. While structural and biochemical studies identified this EGF-induced allosteric activation
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