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Safety Information

77857

Sigma-Aldrich

Ketoreductase

recombinant, expressed in E. coli, ≥0.2 U/mg

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CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

powder

specific activity

≥0.2 U/mg

storage temp.

2-8°C

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Application

Ketoreductases are used to study the stereospecificity of ketoreductase domains . They may be used to reduce precursors of α-chloroalcohols, which are synthetic intermediates for various pharmaceutical compounds .

Biochem/physiol Actions

Ketoreductase, Product 77857, reduces 4-chloroacetoacetate with NADPH as a cosubstrate.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which reduces 1 μmol 4-chloroacetoacetate per minute at pH 6.0 and 25°C (cosubstrate NADPH).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Automation Highlights from the Literature
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Journal of Laboratory Automation, 11, 1-6 (2006)
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Tylactone synthase (TYLS) is a modular polyketide synthase that catalyzes the formation of tylactone (1), the parent aglycone precursor of the macrolide antibiotic tylosin. TYLS modules 1 and 2 are responsible for the generation of antidiketide and triketide intermediates, respectively
Carol Larroy et al.
European journal of biochemistry, 269(22), 5738-5745 (2002-11-09)
A new NADP(H)-dependent alcohol dehydrogenase (the YCR105W gene product, ADHVII) has been identified in Saccharomyces cerevisiae. The enzyme has been purified to homogeneity and found to be a homodimer of 40 kDa subunits and a pI of 6.2-6.4. ADHVII shows
Guangming Xiong et al.
Chemico-biological interactions, 178(1-3), 215-220 (2008-11-13)
3Alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni is a key enzyme in the degradation of steroids in the environment. The encoding gene, hsdA, is expressed only at very low levels in the absence of steroids, but undergoes a several fold induction
X Li et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 41(4), 463-468 (2003-01-30)
ANADPH-dependent 2-Oxoaldehyde reductase was isolated and purified from a marine bacteria Bacillus sp. The purification procedure involved ammonium sulfate fractionation and Q Sepharose FF, Hydroxyapatite, Sephadex G-100 column chromatographies. The specific activity of the purified enzyme was increased by 141.1

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