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Merck
CN

77857

Ketoreductase

recombinant, expressed in E. coli, ≥0.2 U/mg

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-823-8
MDL number:
EC Number:
Specific activity:
≥0.2 U/mg
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

form

powder

specific activity

≥0.2 U/mg

storage temp.

2-8°C

Application

Ketoreductases are used to study the stereospecificity of ketoreductase domains . They may be used to reduce precursors of α-chloroalcohols, which are synthetic intermediates for various pharmaceutical compounds .

Biochem/physiol Actions

Ketoreductase, Product 77857, reduces 4-chloroacetoacetate with NADPH as a cosubstrate.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which reduces 1 μmol 4-chloroacetoacetate per minute at pH 6.0 and 25°C (cosubstrate NADPH).

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Roselyne Castonguay et al.
Journal of the American Chemical Society, 130(35), 11598-11599 (2008-08-13)
Tylactone synthase (TYLS) is a modular polyketide synthase that catalyzes the formation of tylactone (1), the parent aglycone precursor of the macrolide antibiotic tylosin. TYLS modules 1 and 2 are responsible for the generation of antidiketide and triketide intermediates, respectively
Automation Highlights from the Literature
Kerstin Thurow and Hilmar Weinmann
Journal of Laboratory Automation, 11, 1-6 (2006)
Q Ye et al.
Proteins, 44(1), 12-19 (2001-05-17)
Pig aldehyde reductase containing the active site mutation tyrosine(50) to phenylalanine has been crystallized in the presence of the cofactor NADP(H) to a resolution of 2.2 A. This structure clearly shows loss of the tyrosine hydroxyl group and no other
Kazumasa Takenaka et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 14(8), 1972-1975 (2005-08-17)
Carbonyl reductase (CBR) is a cytosolic NADPH-dependent oxidoreductase metabolizing prostaglandins, steroids, quinines, and anthracycline antibiotics. Many experimental studies have shown that CBR plays important roles in the regulation of tumor progression, but clinical significance of CBR status remains unclear. Thus
Ossama El-Kabbani et al.
Journal of medicinal chemistry, 48(17), 5536-5542 (2005-08-19)
Structure determination of porcine aldehyde reductase holoenzyme in complex with the potent aldose reductase inhibitor fidarestat was carried out to explain the difference in the potency of the inhibitor for aldose and aldehyde reductases. The hydrogen bonds between the active-site

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