77237
Percoll®
colloidal silica coated with polyvinylpyrrolidone
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About This Item
MDL number:
UNSPSC Code:
12352200
Recommended Products
storage temp.
2-8°C
Application
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Other Notes
A unique density gradient medium designed to separate cells, subcellular particles and larger viruses under mild conditions. Review; Separation of inner and outer membrane vesicles from E.coli
Legal Information
Percoll is a registered trademark of Cytiva
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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S Morein et al.
Analytical biochemistry, 216(1), 47-51 (1994-01-01)
A rapid and simple method for separating and isolating the inner and outer membranes of Escherichia coli is described. Membrane vesicles were prepared either by passing the bacteria through a French press or by conversion of the cells to spheroplasts
Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
H Pertoft et al.
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