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Merck
CN

75926

Oxonol VI

suitable for fluorescence, ≥95% (UV)

Synonym(s):

1,5-Bis(5-oxo-3-propylisoxazol-4-yl)pentamethine oxonol

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About This Item

Empirical Formula (Hill Notation):
C17H20N2O4
CAS Number:
64724-75-0
Molecular Weight:
316.35
NACRES:
NA.32
PubChem Substance ID:
329766766
UNSPSC Code:
12171500
MDL number:
MFCD00210076

Key Documents

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Product Name

Oxonol VI, suitable for fluorescence, ≥95% (UV)

InChI

1S/C17H20N2O4/c1-3-8-14-12(16(20)22-18-14)10-6-5-7-11-13-15(9-4-2)19-23-17(13)21/h5-7,10-11,20H,3-4,8-9H2,1-2H3/b7-5+,10-6+,13-11-

SMILES string

CCCC1=NOC(=O)\C1=C/C=C/C=C/c2c(O)onc2CCC

InChI key

CPHARWHMAGUXDW-PQWGHOHWSA-N

assay

≥95% (UV)

form

powder

solubility

methanol: soluble

fluorescence

λex 523 nm; λem 630 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

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Application

Oxonol VI can be used as a fluorescent potentiometric indicator to detect the membrane potentials in lipid vesicles. Quantitative measurements of the membrane potentials can be easily calibrated using oxonol VI because of its response to membrane potential changes. It is also used as an electric potential-sensitive probe to study membrane vesicles from E. coli cells.

Other Notes

As an optical indicator for membrane potentials in lipid vesicles

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG4170
BCCC4991
BCBV6963
BCBQ8373V
BCBP0937V

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Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Verkhovskaya M
Bio-protocol, 7(9), e2254-e2254 (2017)
Selective electrodiffusion of zinc ions in a Zrt-, Irt-like protein, ZIPB
Lin W, et al.
Journal of biological and chemical chronicles, 285(50), 39013-39020 (2010)
Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H+-ATPase reconstituted into vesicles
Holoubek A, et al.
Biochimica et Biophysica Acta - Biomembranes, 1609(1), 71-79 (2003)
M J Pringle et al.
Membrane biochemistry, 5(3), 225-241 (1984-01-01)
Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential was discharged by oligomycin and/or Cd2+ (a dithiol
P Richter et al.
Journal of plant physiology, 158(1), 35-39 (2002-06-29)
Euglena gracilis, a unicellular photosynthetic flagellate, uses light and gravity as environmental hints to reach and stay in regions optimal for growth and reproduction. The current model of gravitaxis (the orientation with respect to the earth's gravitational field) is based
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