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75543

Sigma-Aldrich

tris-NTA Biotin trifluoroacetate salt solution

≥80.0% (HPLC)

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Synonym(s):
Biotin-tris-NTA trifluoracetic acid salt
Empirical Formula (Hill Notation):
C59H93N11O25S
CAS Number:
1070867-85-4
Molecular Weight:
1388.49
UNSPSC Code:
12352200
PubChem Substance ID:
329766503
NACRES:
NA.32

Quality Level

100

Assay

≥80.0% (HPLC)

form

liquid

storage temp.

2-8°C

SMILES string

OC(CN(CC(O)=O)[C@H](C(O)=O)CCC(N1CCCN(C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CCN(C(CCCCCNC(CCCCCNC(CCCC[C@H](SC2)[C@]3([H])[C@@]2([H])NC(N3)=O)=O)=O)=O)CCCN(C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CC1)=O)=O

InChI

1S/C59H93N11O25S/c71-43(12-3-1-7-21-61-44(72)13-6-5-11-42-55-38(37-96-42)62-59(95)63-55)60-22-8-2-4-14-45(73)64-23-9-24-66(47(75)19-16-40(57(91)92)69(33-51(81)82)34-52(83)84)29-30-67(48(76)20-17-41(58(93)94)70(35-53(85)86)36-54(87)88)26-10-25-65(28-27-64)46(74)18-15-39(56(89)90)68(31-49(77)78)32-50(79)80/h38-42,55H,1-37H2,(H,60,71)(H,61,72)(H,77,78)(H,79,80)(H,81,82)(H,83,84)(H,85,86)(H,87,88)(H,89,90)(H,91,92)(H,93,94)(H2,62,63,95)/t38-,39-,40-,41-,42-,55-/m0/s1

InChI key

ROTBJHGOGUERSI-LNQQGTGFSA-N

Related Categories

Application

Applications

  • Reversible labeling of proteins or cell surfaces
  • Detection and analysis of target molecules
  • Immobilization of proteins, lipids and cells on surfaces
  • Purification and sample preparation of proteins
  • Coupling with microscopic or spectroscopic probes (e.g., fluorophores)
  • Nickel needs to be added prior application.

Features and Benefits

Features

  • A complex of three NiNTA groups ensures high-affinity binding of His-tags
  • Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
  • Protein binding is stochiometric
  • Tris-NTA is available as amino-group or biotin conjugates

Legal Information

This product was produced by Biotechrabbit GmbH. The use of this product for research purposes is covered by a license from QIAGEN Group. No rights to use this product to perform or offer diagnostic, companion diagnostic, commercial testing or other commercial services for money or money′s worth are granted by the supply of this product expressly, by implication or by estoppel.
Should you wish to use this product for any other purpose not covered by this license, please contact QIAGEN Corporate Business Development at bd@qiagen.com.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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