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74092

Sigma-Aldrich

Sodium phosphate monobasic solution

BioUltra, 5 M in H2O

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Synonym(s):
Monosodium phosphate solution, Sodium dihydrogen phosphate, Sodium dihydrogen orthophosphate,Sodium orthophosphate monobasic, Dihydrogen monosodium phosphate, Dihydrogen sodium phosphate
Empirical Formula (Hill Notation):
H2NaO4P
CAS Number:
Molecular Weight:
119.98
Beilstein:
3903772
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Quality Level

form

liquid

concentration

5 M in H2O

impurities

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

3.0-4.5 (25 °C, 5 M in H2O)

solubility

H2O: 5 M, clear, colorless

density

1.40 g/mL at 20 °C

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025
λ: 280 nm Amax: ≤0.02

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic serves as a source of phosphate ions. The use of sodium phosphate monobasic to correct severe hypophosphatemia conditions has been reviewed.

Application

Sodium phosphate monobasic solution has been used as a component of aCSF (artificial cerebrospinal fluid) stock solution for use in the patch-seq analysis of small interneurons. It has also been used as a supplement in the preparation of crude extract of cells for decentralizing cell-free RNA sensing.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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