72485
Nile Red
for microscopy
Synonym(s):
Nile Blue A Oxazone
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About This Item
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Recommended Products
grade
for microscopy
Quality Level
form
crystals
mp
203-205 °C (lit.)
λmax
553 nm
SMILES string
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
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General description
Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.
Application
Nile Red has been used:
- as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
- to label nanoparticles (NPs)
- for lipid analysis
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Influence of process parameters on formation of fibrous materials from dense calcium caseinate dispersions and fat
Manski JM, et al.
Food Hydrocolloids, 22(4), 587-600 (2008)
Importance of intrinsic properties of dense caseinate dispersions for structure formation
Manski JM, et al.
Biomacromolecules, 8(11), 3540-3547 (2007)
Labeling nanoparticles: Dye leakage and altered cellular uptake
Snipstad S, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 91(8), 760-766 (2017)
Protein Gel Staining Methods: An Introduction and Overview
Steinberg TH
Methods in Enzymology, 463, 541-563 (2009)
Analysis of membrane-bound organelles
Fares H and van der Bliek AM
Methods in Cell Biology, 107, 239-263 (2012)
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