Skip to Content
Merck
CN
All Photos(1)

Documents

71183

Sigma-Aldrich

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

Synonym(s):

Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt

Sign Into View Organizational & Contract Pricing


About This Item

Linear Formula:
CH3COONa
CAS Number:
Molecular Weight:
82.03
Beilstein:
3595639
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
39021906
PubChem Substance ID:
NACRES:
NA.25

grade

for luminescence
for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H2O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

SMILES string

[Na+].CC([O-])=O

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Application

Sodium acetate is suitable for use as a buffer in:
  • protein crystallization
  • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
  • the preparation of standard (acetylcholine chloride) for hestrin assay
  • substrate screening characterization for microfluidic microbial fuel cell array

Biochem/physiol Actions

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

O-acetylation of typhoid capsular polysaccharide confers polysaccharide rigidity and immunodominance by masking additional epitopes
Hitri K, et al.
Vaccine (2019)
Microfluidic microbial fuel cell array for multiplexed long-term parallel analysis of microbial activities
Hou H, et al.
15th international conference on miniaturized systems for chemistry and life sciences, Seattle, Washington (2011)
Enzyme Crystals and Hydrogel Composite Membranes as New Active Food Packaging Material
Mirabelli V, et al.
Global challenges (Hoboken, NJ) (2018)
Determination of the G+ C content of prokaryotes
Mesbah NM, et al.
Methods in Microbiology (2011)
Large- and small-scale phenol extractions.
D M Wallace
Methods in enzymology, 152, 33-41 (1987-01-01)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service