All Photos(1)
Synonym(s):
2-(4-morpholino)ethanesulfonic acid, 2-morpholinoethanesulfonic acid, Morpholine-4-ethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
Linear Formula:
C6H13NO4S · H2O
CAS Number:
Molecular Weight:
213.25
Beilstein:
6350956
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
product line
BioXtra
Quality Level
Assay
≥99.0% (T)
form
powder or crystals
useful pH range
5.5-6.7
pKa (25 °C)
6.1
mp
>300 °C (lit.)
solubility
H2O: 0.5 M at 20 °C, clear
density
10.66 g/mL
anion traces
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
cation traces
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg
application(s)
agriculture
diagnostic assay manufacturing
general analytical
microbiology
foreign activity
DNase, none detected
NICKase, none detected
RNase, none detected
protease, none detected
SMILES string
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
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General description
MES monohydrate, also known as 2-Morpholinoethanesulfonic acid hydrate, is a buffer used in research applications in biology and biochemistry. MES is a pH buffering compound with a pKa value of 6.15 at 20 oC. MES was developed in the 1960s as one of Good′s buffers, which were designed with various criteria in mind, including midrange pKa, maximum water solubility, minimum solubility in all other solvents, and minimal change in pKa with temperature. MES is highly soluble in water and only weakly binds with certain metal ions, including Ca, Mg, Mn, and Cu(II), which makes it a non-coordinating buffer in chemistry involving metal ions. Further, It plays a crucial role in maintaining a stable environment in various applications, including cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES monohydrate finds extensive use in purifying antibodies, peptides, proteins, blood components, and growth factors. It also stands out as an excellent choice for capillary electrochromatography due to its low ionic mobility, offering a safer alternative to toxic compounds like cacodylate and non-zwitterionic buffers such as citrate and malate. Additionally, MES Monohydrate serves various purposes, including pH adjustment in growth media, stabilizing enzymatic solutions, and as a component of PAGE running buffers in diverse experimental settings.
Application
MES Monohydrate has been used:
- to adjust the pH of the growth medium, facilitating gene expression analyses aimed at understanding the molecular mechanisms
- in the fixation and imaging of cellular components, enabling the study of hypotonic stress′s physiological effects on cell structure and function through Super-Resolution and Live-Cell Microscopy
- to adjust the pH of the growth medium to 5.5-7.0 in an in vitro cell wall stress assay
- as a buffering agent to stabilize enzymatic solutions
- as a component of PAGE running buffer
Features and Benefits
- Suitable for Biochemical and Cell Biology Research
- Tested for the presence of anionic and cationic traces
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
Preparation Note
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Other Notes
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
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In vitro cell wall stress assay for Fusarium oxysporum.
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Bio-protocol, 6, e1915-e1915 (2016)
Dawson, R.M.C. et al.
Data for Biochemical Research, 410- 410 (1987)
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