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69554

Sigma-Aldrich

Peptidoglycan from Bacillus subtilis

Synonym(s):

PGN

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About This Item

MDL number:
MFCD00212486
UNSPSC Code:
41116134
NACRES:
NA.21

form

solid

Quality Level

100

storage temp.

−20°C

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General description

Peptidoglycan.is a biopolymer that is a major component of the cell walls of Gram-positive bacteria. Peptidoglycan consists of a carbohydrate backbone of alternating units of N-acetyl glucosamine and N-acetyl muramic acid and is modified with both D- and L-amino acids. N-Acetyl muramic acid residues are cross-linked with oligopeptides. The terminal peptide is D-alanine although other amino acids are present as D-isomers.

Application

Peptidoglycan may be used for the activity estimation of lytic enzymes (e.g. lyticase). It is recommended to use a peptidoglycan concentration of 0.15-3 μg/ml in water or buffer and measuring at 450 nm. The peptidoglycan is not soluble but produces a suspension in water.
Peptidoglycan activates the Toll-like receptor 2 (TLR2), present in mammalian cells for the stimulation of lymphocytes. It may also function as an antagonist of Poly (I:C).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000322617
0000322617
0000256007
0000256007
0000137174

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product 69554, Peptidoglycan, Gram positive or Gram negative?

    Peptidoglycan is Gram positive.

  6. What is the method of preparation of Product 69554, Peptidoglycan?

    Peptidoglycan is made by fermentation and so it is from vegetative cell bacteria.  Peptidoglycan is from strain DSM 10.

  7. What is the molecular weight of Product 69554, Peptidoglycan?

    Unfortunately, we have no references reporting the molecular weight of peptidoglycan.  Because it is not soluble without destroying the original structure, the molecular weight can not be determined by suitable analytical methods (GPC, viscosity, light scattering).

  8. What is the solubility of Product 69554, Peptidoglycan?

    Peptidoglycans are insoluble in all solvents.  To increase the recovery of solubility, let the peptidoglycan soak in an aqueous buffer for several hours or overnight.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Articles

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

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