Skip to Content
Merck
CN

68957

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, suitable for electrophoresis, SDS-PAGE tested

Synonym(s):

TCEP.HCL, TCEP

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C9H15O6P · HCl
CAS Number:
51805-45-9
Molecular Weight:
286.65
UNSPSC Code:
12352128
NACRES:
NA.32
PubChem Substance ID:
329761275
MDL number:
MFCD00145469
Beilstein/REAXYS Number:
3724376

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Tris(2-carboxyethyl)phosphine hydrochloride, BioUltra, suitable for electrophoresis, SDS-PAGE tested

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

product line

BioUltra

assay

97.5-102.5%

form

powder

Quality Level

100

mol wt

286.65 g/mol

concentration

90-100 % (w/w) (Tris(2-carboxyethyl)phosphine hydrochloride)

technique(s)

electrophoresis: suitable

color

white

mp

175-177 °C

solubility

H2O: soluble

density

1.041 g/cm3 at 25 °C

suitability

SDS-PAGE tested

Looking for similar products? Visit Product Comparison Guide

Related Categories

Analysis Note

agarose gel electrophorese corresponds to requirements

Application

TCEP.HCL has been used in various bioconjugation applications, both in vitro and in vivo, to tag proteins and live organisms with great effectiveness under mild physiological circumstances. TCEP.HCL is suitable for varied biological and synthetic applications. At an acidic pH, TCEP.HCL can be used to convert organic disulfides to thiols in water irreversibly.
Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Features and Benefits

The product has the following benefits:

  • TCEP.HCL lowers reactive oxygen species levels.
  • It increases glutathione levels and mitochondrial content.
  • It has anti-cancer effects.

General description

Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCL ) is a useful reducing agent for Sulphur and nitrogen-containing compounds. TCEP.HCL and the resultant oxide are water-soluble. TCEP.HCL has electron-rich phosphorus and oxygen atoms to combine with various metals, including Rh, Ir, and Pd. It rapidly complexes with Rh and Ir in dioxane at ambient temperature. It reduces disulfide linkages in a range of peptides and proteins in biological disulfide.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN6353
BCCM5857
BCCH3116
BCCG6486
BCCG0947

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Christopher R Schlieve et al.
Experimental eye research, 83(5), 1252-1259 (2006-08-29)
Retinal ganglion cells (RGCs) undergo apoptosis after axonal injury, in part regulated by an intracellular superoxide anion burst, for which the target(s) are unknown. Shifting the RGC redox state towards reduction and preventing sulfhydryl oxidation is neuroprotective in vitro and
Yiren Zeng et al.
Theriogenology, 162, 32-41 (2021-01-15)
Oocyte in vitro maturation (IVM) is a crucial process that determines subsequent in vitro embryo production. The present study investigated the effects of the antioxidant tris (2-carboxyethyl) phosphine hydrochloride (TCEP-HCL) on the in vitro maturation of porcine oocytes and in vitro developmental competence of
W H Fischer et al.
Rapid communications in mass spectrometry : RCM, 7(3), 225-228 (1993-03-01)
The suitability of the hydrochloride salt of tris(2-carboxyethyl)phosphine (TCEP) for in situ reduction with matrix-assisted laser desorption/ionization (MALDI) and liquid secondary ionization (LSI) mass spectrometry is evaluated. TCEP can be used to irreversibly reduce organic disulfides to thiols in water
Siming Chen et al.
Chemical communications (Cambridge, England), 49(12), 1226-1228 (2013-01-05)
A widely used reducing agent tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein. This discovery clarifies the reactivity of cisplatin towards Sp1 zinc finger protein and implies that the reactions of platinum drugs could be
Fang-Fang Cheng et al.
The Analyst, 139(16), 3860-3865 (2014-07-01)
The expression of microRNAs (miRNAs) is related to some cancer diseases. Recently, miRNAs have emerged as new candidate diagnostic and prognostic biomarkers for detecting a wide variety of cancers. Due to low levels, short sequences and high sequence homology among
View All Related Papers

Articles

Quality Attribute Determination of Monoclonal Antibodies

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

Protocols

Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

High-Throughput Glycoprofiling Workflow for Rituximab

SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service