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67455

Sigma-Aldrich

Atto 514 NHS ester

suitable for fluorescence

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1 ML
CN¥1,812.10

CN¥1,812.10

Available to ship onApril 16, 2025Details

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1 ML
CN¥1,812.10

About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

CN¥1,812.10

Available to ship onApril 16, 2025Details

Request a Bulk Order

Assay

90% (Coupling efficiency with benzylamine, HPLC)

form

solid

mol wt

Mw 1111 g/mol

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
514 nm

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

λ

(water/methanol with 0.1% perchloric acid)

suitability

suitable for fluorescence

storage temp.

−20°C

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application(s)

cannabis testing
cannabis testing

application(s)

cannabis testing
cannabis testing

application(s)

cannabis testing
cannabis testing

application(s)

cannabis testing
cannabis testing

form

liquid

form

liquid

form

liquid

form

liquid

format

single component solution

format

single component solution

format

single component solution

format

single component solution

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

General description

Atto 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield and exceptional thermal and photo-stability. Thus Atto 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of the Argon-Ion laser.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM9229
    BCCL5721
    BCCK3342
    BCCK1790
    BCCJ9352

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    Hsin-Yi Hsieh et al.
    ACS nano, 13(4), 4486-4495 (2019-03-12)
    Interest in developing a rapid and robust DNA sequencing platform has surged over the past decade. Various next-/third-generation sequencing mechanisms have been employed to replace the traditional Sanger sequencing method. In sequencing by synthesis, a signal is monitored by a

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