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Sigma-Aldrich

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

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Synonym(s):
Ethyl Green
Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
Colour Index Number:
42590
Beilstein:
3914106
EC Number:
230-415-4
MDL number:
MFCD00151094
UNSPSC Code:
12171500
PubChem Substance ID:
329760277
NACRES:
NA.47

grade

for microscopy (Bact., Bot., Hist.)

Quality Level

200

form

powder

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥900 at 624-634 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Biochem/physiol Actions

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Analysis Note

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.

Signal Word

Danger

Hazard Statements

H314,H335,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Monocyte chemoattractant protein-1 in human atheromatous plaques.
Nelken NA, et al.
The Journal of Clinical Investigation, 88, 1121-1121 (1991)
K R Fox et al.
European journal of histochemistry : EJH, 36(3), 263-270 (1992-01-01)
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At
Evaluation of apoptosis as a mechanism of follicular cell atresia in the ovaries of cattle (bos indicus) and buffalo (bubalus bubalis) fetuses.
Santos SSD, et al.
Animal Reproduction, 10, 55-61 (2013)
Chun-Chi Liang et al.
The Journal of clinical investigation, 124(7), 3080-3092 (2014-06-18)
Lack of a preclinical model of primary dystonia that exhibits dystonic-like twisting movements has stymied identification of the cellular and molecular underpinnings of the disease. The classical familial form of primary dystonia is caused by the DYT1 (ΔE) mutation in
Jaklien C Leemans et al.
PloS one, 4(5), e5704-e5704 (2009-05-30)
Tissue fibrosis and chronic inflammation are common causes of progressive organ damage, including progressive renal disease, leading to loss of physiological functions. Recently, it was shown that Toll-like receptor 2 (TLR2) is expressed in the kidney and activated by endogenous
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