Skip to Content

很抱歉，目前系统维护中。在此期间，网站下单功能无法使用。给您带来不便，深表歉意。如需其他帮助，请联系客户联络中心：400 620 3333。

Merck
CN
All Photos(1)

Key Documents

View All Documentation

Safety Information

64958

Sigma-Aldrich

2-Methoxy-2,4-diphenyl-3(2H)-furanone

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

Sign Into View Organizational & Contract Pricing

Select a Size

25 MG
¥1,932.63
100 MG
¥5,832.80

¥1,932.63

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(1)

Select a Size

Change View
25 MG
¥1,932.63
100 MG
¥5,832.80

About This Item

Empirical Formula (Hill Notation):
C17H14O3
CAS Number:
50632-57-0
Molecular Weight:
266.29
Beilstein:
1288529
MDL number:
MFCD00036834
UNSPSC Code:
12352108
PubChem Substance ID:
329760016
NACRES:
NA.32

¥1,932.63

Please contact Customer Service for Availability
Request a Bulk Order

Assay

≥98.0% (HPLC)

form

solid

fluorescence

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

Application

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Other Notes

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed[1][2]; Pre-column derivatization of amines[3]; Fluorescent labeling of proteins before SDS-PAGE[4]

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCL1534
    BCBT5586
    BCBS6087V
    BCBP1051V
    BCBK6273V

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    F J Alba et al.
    Electrophoresis, 18(11), 1960-1966 (1998-01-07)
    We have studied the light emission efficiency of proteins labeled with different fluorescent dyes chemically excited by the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 reaction. Using this peroxyoxalate chemiluminescence system, the best results were obtained with proteins covalently labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF). Blotted proteins
    M Hengstschläger et al.
    Cytometry, 14(1), 39-45 (1993-01-01)
    Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for diagnosis. Although of great interest, its regulation during cell cycle and differentiation is difficult to study, as current techniques for isolation of cells in different
    J C Stockert et al.
    Blood cells, 19(2), 423-430 (1993-01-01)
    The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this work, we describe the application of
    V Toome et al.
    Biochemical and biophysical research communications, 114(2), 433-439 (1983-07-29)
    2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) reacts readily with the free amino group of a dipeptide to form a pyrrolinone-type chromophore with absorption maxima at 275-285 and 370-390 nm. A simple test tube procedure is described which allows in situ correlation of the absolute
    V E Urwin et al.
    Analytical biochemistry, 195(1), 30-37 (1991-05-15)
    A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tubes for the isoelectric focusing
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service