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64010

Sigma-Aldrich

Metanil Yellow

for microscopy (Hist.), indicator (pH 1.2-2.3)

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Synonym(s):
3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36
Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
587-98-4
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
209-608-2
MDL number:
MFCD00007486
UNSPSC Code:
12171500
PubChem Substance ID:
329759919
NACRES:
NA.47

grade

for microscopy (Hist.)
indicator (pH 1.2-2.3)

form

powder

solubility

methanol: 0.01 g/10 mL, orange to very deep orange

εmax

≥500 at 409-419  nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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An improved stain for connective tissue cells in plastic-embedded sections.
Laboratory Medicine, 10, 783-785 (1979)
Photocatalytic degradation of azo dye Metanil Yellow: optimization and kinetic modeling using a chemometric approach.
Applied Catalysis. B, Environmental, 77, 1-11 (2007)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)
P B Rastogi et al.
Journal of food protection, 59(3), 244-248 (1996-03-01)
Among a variety of experimental protocols used, the combined use of 0.5% dextrose in bottom agar and 1 micromol of flavin mononucleotide (FMN) in preincubation mixtures without fraction S9 mix resulted in the highest level of induced Salmonella typhimurium his
Edgar J Ruiz et al.
Chemosphere, 82(4), 495-501 (2010-11-30)
The degradation of the Acid Yellow 36 (AY36) azo dye is studied by electro-Fenton (EF) and solar photoelectro-Fenton (SPEF) using a recirculation flow plant with an undivided cell containing a boron-doped diamond anode and an air-diffusion cathode for H₂O₂ electrogeneration
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