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Merck
CN

64010

Metanil Yellow

suitable for microscopy

Synonym(s):

3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36

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About This Item

Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
587-98-4
Molecular Weight:
375.38
EC Number:
209-608-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329759919
MDL number:
MFCD00007486
Beilstein/REAXYS Number:
3831568

Key Documents

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InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

form

powder

solubility

methanol: 0.01 g/10 mL, orange to very deep orange

εmax

≥500 at 409-419  nm in methanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4503
BCCC0847
BCCB6873
BCBQ7911V
BCBP5613V

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Photocatalytic degradation of azo dye Metanil Yellow: optimization and kinetic modeling using a chemometric approach.
Applied Catalysis. B, Environmental, 77, 1-11 (2007)
An improved stain for connective tissue cells in plastic-embedded sections.
Laboratory Medicine, 10, 783-785 (1979)
Cellular localization of latent murine cytomegalovirus.
Koffron AJ, et al.
Journal of Virology, 72, 95-95 (1998)
Saijun Lin et al.
Se pu = Chinese journal of chromatography, 29(1), 79-82 (2011-05-18)
A method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed for simultaneous determination of five yellow industrial dyes. The separations were performed with an Agilent ODS C18 column at a flow rate of 0.3
M Das et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 35(8), 835-838 (1997-08-01)
Metanil yellow, a non-permitted food colour, has been found in various foodstuffs. The induction potential of metanil yellow on hepatic microsomal cytochrome P-450 (P-450)-dependent monooxygenases and cytosolic detoxification enzymes, namely, glutathione S-transferase (GST) and quinone reductase (QR), was investigated. Oral
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