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Sigma-Aldrich

LR Accelerator

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MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

impurities

≥20% N,N-dimethyl-p-toluidine

density

1.050 g/cm3

application(s)

hematology
histology

storage temp.

room temp

Application

LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.

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Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2 Oral

Target Organs

Reproductive organs

WGK

WGK 3

Flash Point(F)

168.8 °F

Flash Point(C)

76 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Margarita Sobol et al.
Histochemistry and cell biology, 134(6), 631-641 (2010-11-11)
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration
Shuji Yamashita
Methods in molecular biology (Clifton, N.J.), 657, 237-248 (2010-07-06)
We describe a standardized method of fixation, antigen retrieval, and image contrasting for post-embedding immunoelectron microscopy. Tissues are fixed with formaldehyde solutions containing Ca(2+) and Mg(2+) ions at pH 7.4 and then at pH 8.5. After dehydration with dimethylformamide, the
Margarita Sobol et al.
Histochemistry and cell biology, 135(1), 103-110 (2010-12-15)
The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still
M Giagnacovo et al.
European journal of histochemistry : EJH, 54(3), e31-e31 (2010-09-08)
The aim of the present investigation was to evaluate whether routinely frozen biopsies of human skeletal muscle may be suitable for morphological and immunocytochemical analyses at transmission electron microscopy. The fixation/embedding protocols we successfully used for decades to process fresh
C J von Ruhland et al.
Journal of microscopy, 240(2), 130-134 (2010-10-16)
The utility of LR White sections as slot grid support films for the examination of thin resin-embedded tissue sections by transmission electron microscopy was investigated and compared with traditional formvar-carbon films. Throughout a variety of staining procedures, which involved the

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