impurities
≥20% N,N-dimethyl-p-toluidine
density
1.050 g/cm3
application(s)
hematology
histology
storage temp.
room temp
Application
LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - Carc. 1B - Repr. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Reproductive organs
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
168.8 °F
flash_point_c
76 °C
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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C J von Ruhland et al.
Journal of microscopy, 240(2), 130-134 (2010-10-16)
The utility of LR White sections as slot grid support films for the examination of thin resin-embedded tissue sections by transmission electron microscopy was investigated and compared with traditional formvar-carbon films. Throughout a variety of staining procedures, which involved the
Margarita Sobol et al.
Histochemistry and cell biology, 134(6), 631-641 (2010-11-11)
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration
Shuji Yamashita
Methods in molecular biology (Clifton, N.J.), 657, 237-248 (2010-07-06)
We describe a standardized method of fixation, antigen retrieval, and image contrasting for post-embedding immunoelectron microscopy. Tissues are fixed with formaldehyde solutions containing Ca(2+) and Mg(2+) ions at pH 7.4 and then at pH 8.5. After dehydration with dimethylformamide, the
Olivier Gros et al.
Acta histochemica, 110(5), 427-431 (2008-01-12)
Hydrophilic resins present the advantage of making possible both hybridization experiments involving either antibodies or oligonucleotide probes and ultrastructural observations. Whereas various embedding protocols are available, only very few concern flat-embedded preparations. In this study we describe an easy protocol
Vendula Strádalová et al.
Histochemistry and cell biology, 130(5), 1047-1052 (2008-09-18)
A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded
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