62310
Lipase from Rhizopus niveus
powder (fine), ≥1.5 U/mg
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Triacylglycerol acylhydrolase, Triacylglycerol lipase
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biological source
fungus (Rhizopus niveus)
Quality Level
form
powder (fine)
specific activity
≥1.5 U/mg
mol wt
Mr ~83000
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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General description
Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.
Application
Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.
Biochem/physiol Actions
Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.
Other Notes
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Simulation of gastric lipolysis and prediction of felodipine release from a matrix tablet in the fed stomach
European Journal of Pharmaceutical Sciences (2009)
Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae
Microbiology (2016)
Purification, characterization and crystallization of two types of lipase from Rhizopus niveus
Bioscience, Biotechnology, and Biochemistry, 58(6) (1994)
Food Biotechnology (1995)
Synlett, 105-105 (1990)
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