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Merck
CN

61305

Penicillinase from Bacillus cereus

lyophilized, powder, white, ~13 U/mg

Synonym(s):

β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase

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About This Item

CAS Number:
9073-60-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819

Key Documents

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Product Name

Penicillinase from Bacillus cereus, lyophilized, powder, white, ~13 U/mg

biological source

Bacillus sp. (Bacillus cereus)

form

powder

quality

lyophilized

specific activity

~13 U/mg

mol wt

Mr ~30000

technique(s)

activity assay: suitable

color

white

suitability

suitable for component for culture media

application(s)

diagnostic assay manufacturing

foreign activity

β-lactamase II ≤2%

storage temp.

−20°C

Quality Level

100

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Application

Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.
Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

General description

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

Other Notes

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL1159
BCCM2918
BCCK6124
BCCJ0566
BCCD7047

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P K Pandey et al.
Clinica chimica acta; international journal of clinical chemistry, 190(3), 175-184 (1990-10-15)
An enzyme-linked immunosorbant assay (ELISA) for measuring oestradiol directly in plasma without extraction utilizing antibodies raised against oestradiol-3-(O-carboxymethyl) ether-bovine serum albumin conjugate, and oestradiol-6-(O-carboxymethyl) oxime linked to penicillinase (EC 3.5.2.6) as a marker was developed. Polyvinyl 96-well microtitre plates were
Properties of penicillinase from Bacillus cereus 569
Imsande J, et al.
The Journal of Biological Chemistry, 245(9), 2205-2212 (1970)
T. Scheper et al.
Analytica Chimica Acta, 163, 111-111 (1984)
Flow injection determination of penicillins using immobilized penicillinase in a single bead string reactor.
R Gnanasekaran et al.
Analytical chemistry, 57(6), 1005-1009 (1985-05-01)
W. Hobel et al.
Biosensors And Bioelectronics, 7, 549-549 (1992)
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