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Safety Information

59205C

SAFC

HEPES Buffer Solution (1M)

Synonym(s):

HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

CAS Number:
7365-45-9
MDL number:
MFCD00006158
UNSPSC Code:
12352207
PubChem Substance ID:
329758606

description

for research or for further manufacturing use

Quality Level

300

form

liquid

useful pH range

6.8-8.2

storage temp.

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Application

HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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