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GS Supplement 50X

50 ×, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

Synonym(s):

GSEM Supplement, Glutamine synthetase expression medium supplement

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About This Item

MDL number:
MFCD00677096
UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

Quality Level

500

sterility

sterile-filtered

form

liquid

concentration

50 ×

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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General description

The GS System™ uses the Glutamine synthetase (GS) gene as a selectable marker for the expression of recombinant proteins in mammalian cells, especially NS0 and CHO cells. The system has been shown to have significant advantages with respect to speed and efficiency of cell line development and has resulted in high productivity for antibodies and other recombinant proteins.

Application

GS Supplement 50X has been used to as a supplement for culture media to grow GS-NS0 mouse myeloma cells.
The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Storage and Stability

Store GS Supplement 50X protected from light at -10 to -40 °C.

Legal Information

GS System is a trademark of Lonza Group Ltd.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Energy-based culture medium design for biomanufacturing optimization: A case study in monoclonal antibody production by GS-NS0 cells.
Quiroga-Campano A L, et al.
Metabolic engineering, 47, 21-30 (2018)
Doyle, A., et al.
Cell and Tissue Culture: Laboratory Procedures, 27D, 3-3 (1999)

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