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Merck
CN

58030

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym(s):

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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About This Item

Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
146-68-9
Molecular Weight:
505.70
UNSPSC Code:
12352102
NACRES:
NA.25
PubChem Substance ID:
57651636
EC Number:
205-676-2
Beilstein/REAXYS Number:
4093224
MDL number:
MFCD00011961

Key Documents

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Product Name

Iodonitrotetrazolium chloride, BioReagent, ≥97.0% (calc. on dry substance, NT)

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

product line

BioReagent

assay

≥97.0% (calc. on dry substance, NT)

mp

240 °C (dec.) (lit.)
~245 °C (dec.)

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

application(s)

histology

Quality Level

200

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Application

Electron acceptor for the colorimetric assay of various dehydrogenases
Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

hcodes

H228,H335

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM1565
BCCF6258
BCCD3777
BCBT1019
BCBQ7328V

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