Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

57180

Sigma-Aldrich

Indo 1

for fluorescence, ≥80.0% (HPLC)

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C32H26K5N3O12
CAS Number:
132319-56-3
Molecular Weight:
840.05
MDL number:
MFCD00077797
UNSPSC Code:
12352200
PubChem Substance ID:
329758276
NACRES:
NA.32

grade

for fluorescence

Assay

≥80.0% (HPLC)

form

solid

fluorescence

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+
λex 349 nm; λem 482 nm (100mM KCI)

suitability

in accordance for fluorescence

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChI key

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Wanwarang Wongcharoen et al.
Cardiovascular research, 70(3), 497-508 (2006-04-01)
Pulmonary veins (PVs) are the most important focus for generation of atrial fibrillation. The Na(+)/Ca(2+) exchange (NCX) current is important in PV electrical activity and cardiac glycosides-induced arrhythmias. The purpose of this study was to investigate whether KB-R7943, a NCX
Yael Yaniv et al.
PloS one, 7(5), e37582-e37582 (2012-06-06)
Mitochondria dynamically buffer cytosolic Ca(2+) in cardiac ventricular cells and this affects the Ca(2+) load of the sarcoplasmic reticulum (SR). In sinoatrial-node cells (SANC) the SR generates periodic local, subsarcolemmal Ca(2+) releases (LCRs) that depend upon the SR load and
K Nishimaru et al.
British journal of pharmacology, 152(4), 456-463 (2007-07-21)
The potent vasoconstrictor polypeptide endothelin-1 (ET-1) plays an important pathophysiological role in progression of cardiovascular diseases and elicits prominent effects on myocardial contractility. Although ET-1 produces a positive inotropy in cardiac muscle of most mammalian species, it induces a sustained
Terence R Moopanar et al.
The Journal of physiology, 571(Pt 1), 191-200 (2005-12-13)
The aim of this study was to further characterize the reduction of myofibrillar Ca2+ sensitivity in mouse muscle which has been observed after fatigue at 37 degrees C. Muscle bundles and single fibres were isolated from mouse flexor digitorum brevis
Sandrine Pouvreau et al.
The Journal of physiology, 567(Pt 3), 815-828 (2005-07-05)
Nitric oxide (NO) generated by skeletal muscle is believed to regulate force production but how this is achieved remains poorly understood. In the present work we tested the effects of NO synthase (NOs) inhibitors on membrane current and intracellular calcium
View All Related Papers

Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service