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Merck
CN

57180

Indo 1

suitable for fluorescence, ≥80.0% (HPLC)

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt

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About This Item

Empirical Formula (Hill Notation):
C32H26K5N3O12
CAS Number:
132319-56-3
Molecular Weight:
840.05
NACRES:
NA.32
PubChem Substance ID:
329758276
UNSPSC Code:
12352200
MDL number:
MFCD00077797

Key Documents

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Product Name

Indo 1, suitable for fluorescence, ≥80.0% (HPLC)

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI key

VGZPRMSXVNDMAN-UHFFFAOYSA-I

assay

≥80.0% (HPLC)

form

solid

fluorescence

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+
λex 349 nm; λem 482 nm (100mM KCI)

suitability

in accordance for fluorescence
suitable for fluorescence

storage temp.

−20°C

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB7609
BCBV4049
BCBQ7736V
BCBK8919V

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Sandrine Pouvreau et al.
The Journal of physiology, 567(Pt 3), 815-828 (2005-07-05)
Nitric oxide (NO) generated by skeletal muscle is believed to regulate force production but how this is achieved remains poorly understood. In the present work we tested the effects of NO synthase (NOs) inhibitors on membrane current and intracellular calcium
Microsphere-based real-time calcium sensing.
Rosario M Sánchez-Martín et al.
Angewandte Chemie (International ed. in English), 45(33), 5472-5474 (2006-07-19)
K G Lazzari et al.
The Journal of biological chemistry, 261(21), 9710-9713 (1986-07-25)
The activation of human neutrophils by chemotactic peptides evokes a rapid change in membrane potential and an increase in cytoplasmic Ca2+ levels. These events are followed up to a minute later by detectable levels of microbicidal agents formed by the
M K Bijsterbosch et al.
Biochemical and biophysical research communications, 137(1), 500-506 (1986-05-29)
The new Ca2+-probe indo-1 has a high fluorescence intensity, which allows low intracellular dye loadings. Stimulation of indo-1-loaded mouse B cells with anti-Ig antibodies provoked rapid rise of free cytoplasmic Ca2+ from 100 nM to greater than 1 microM, followed
Michael Bannwarth et al.
ACS chemical biology, 4(3), 179-190 (2009-02-06)
The role of calcium in signal transduction relies on the precise spatial and temporal control of its concentration. The existing means to detect fluctuations in Ca2+ concentrations with adequate temporal and spatial resolution are limited. We introduce here a method
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荧光寿命测量

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